E-RNAi is a tool for the design and evaluation of RNAi reagents for a variety of species. It can be used to design and evaluate long dsRNAs (including esiRNAs) as well as siRNAs. Version 3.2 of E-RNAi now offers batch queries as wells as sequence submission from GBrowse.
To get started, please choose the sequence selection option (input of gene identifier or sequence), the GBrowse submission option or the reagent evaluation option.
Full documentation of all query options, settings and explanations of outputs is available in the E-RNAi Wiki.
Reference
E-RNAi: a web application for the multi-species design of RNAi reagents--2010 update.
Thomas Horn; Michael Boutros
Nucleic Acids Research May 5, 2010. [link to article].